CMSP offers mass spectrometry services for detection of post-translational modifications (PTM’s), including amino acid site localization on proteins and PTM quantification. The workflows for the services vary based on the type of PTM under investigation. The common component of the PTM investigations is the method by which the PTM’s are detected: ‘bottom-up’ (peptide-level) detection on a high resolution mass spectrometer.
CMSP accepts protein samples for phosphopeptide enrichment. Proteins will be proteolytically digested and enriched at the peptide level with a dual enrichment protocol, specifically the High-Slect(™) SMOAC (Sequential enrichment of Metal Oxide Affinity Chromatography) method by ThermoFisher Scientific. SMOAC includes titanium dioxide (TiO2) enrichment followed by FeNTA enrichment of the flow through from the TiO2 procedure. Each peptide pool is analyzed by LC-tandem mass spectrometry on a capillary column.
CMSP accepts protein samples for proteolytic digestion and sample clean-up prior to peptide-level Acetylation and Ubiquitin enrichment in the lab of the research customer. After the PTM enrichment process is completed, samples can be resubmitted to CMSP for LC-tandem mass spectrometry on a capillary column.
CMSP will work with customers to establish workflows for additional modifications that are within the realm of the resources at the core.