CMSP offers the latest technologies for quantitative proteomic analyses. Evaluate potential biomarkers among thousands of protein and peptide signatures among your experimental conditions using a label-free or stable isotope labeled approach. Don’t know which to choose? Our proteomics team offers complimentary consultations to help you choose the best approach for your experimental design.
Quantification of relative protein/peptide abundance from precursor signal intensity. A LFQ design is our most flexible analysis strategy and can accommodate as many observations as your experimental design requires. Running each sample sequentially, protein and peptide abundances are matched between injections and the relative abundance of peptide precursors are compared between experimental conditions. Note - LFQ analysis is not compatible with our offline-fraction techniques to maximize proteome coverage.
Using isobaric mass tagging reagents, experiments may be multiplexed and multiple observations analyzed simultaneously. Sample quantification is obtained directly from a nominal mass tag removing the need to perform retention time alignment. In addition to an increased quantitative precision, TMT labeling enables the use of offline-fractionation techniques to maximize proteomic coverage. CMSP offers analyses using the latest Thermo Scientific™ TMT products, including TMT 10plex and TMTpro™ 16plex.
Analyze cell cultures grown in light- or heavy-labeled growth medium in a multiplexed LC-MS/MS experiment. SILAC methodology enables specialized experimental designs and in-depth analysis of cellular dynamics. Our software deconvolutes light and heavy peptide signals and provides you quantitative measurements of thousands of proteins using a shotgun proteomics approach.